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Plos One : Rapid Discrimination of Haemophilus Influenzae, H. Parainfluenzae, and H. Haemolyticus by Fluorescence in Situ Hybridization Fish and Two Matrix-assisted Laser- Desorption-ionization Time-of-flight Mass Spectrometry Maldi-tof-ms Platforms, Volu

By Kashanchi, Fatah

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Book Id: WPLBN0003965659
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Reproduction Date: 2015

Title: Plos One : Rapid Discrimination of Haemophilus Influenzae, H. Parainfluenzae, and H. Haemolyticus by Fluorescence in Situ Hybridization Fish and Two Matrix-assisted Laser- Desorption-ionization Time-of-flight Mass Spectrometry Maldi-tof-ms Platforms, Volu  
Author: Kashanchi, Fatah
Volume: Volume 8
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
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Publisher: Plos

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Kashanchi, F. (n.d.). Plos One : Rapid Discrimination of Haemophilus Influenzae, H. Parainfluenzae, and H. Haemolyticus by Fluorescence in Situ Hybridization Fish and Two Matrix-assisted Laser- Desorption-ionization Time-of-flight Mass Spectrometry Maldi-tof-ms Platforms, Volu. Retrieved from http://kindle.worldlibrary.net/


Description
Description : Methodology : A strain collection of 84 Haemophilus spp. comprising 50 H. influenzae, 25 H. parainfluenzae, 7 H. haemolyticus, and 2 H. parahaemolyticus including 77 clinical isolates was analyzed by FISH with newly designed DNA probes, and two different MALDI-TOF-MS systems (Bruker, Shimadzu) with and without prior formic acid extraction. Principal Findings : Among the 84 Haemophilus strains analyzed, FISH led to 71 correct results (85%), 13 uninterpretable results (15%), and no misidentifications. Shimadzu MALDI-TOF-MS resulted in 59 correct identifications (70%), 19 uninterpretable results (23%), and 6 misidentifications (7%), using colony material applied directly. Bruker MALDI-TOF-MS with prior formic acid extraction led to 74 correct results (88%), 4 uninterpretable results (5%) and 6 misidentifications (7%). The Bruker MALDI-TOF-MS misidentifications could be resolved by the addition of a suitable H. haemolyticus reference spectrum to the system’s database. In conclusion, no analyzed diagnostic procedure was free of errors. Diagnostic results have to be interpreted carefully and alternative tests should be applied in case of ambiguous test results on isolates from seriously ill patients.

 

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